Three dimensional particle averaging for structural imaging of macromolecular complexes by localization microscopy
Published in biorxiv, 2019
Recommended citation: Hamidreza, Heydarian et al. (2019). "Three dimensional particle averaging for structural imaging of macromolecular complexes by localization microscopy." biorxiv. https://www.biorxiv.org/content/10.1101/837575v1
We present an approach for 3D particle fusion in localization microscopy which dramatically increases signal-to-noise ratio and resolution in single particle analysis. Our method does not require a structural template, and properly handles anisotropic localization uncertainties. We demonstrate 3D particle reconstructions of the Nup107 subcomplex of the nuclear pore complex (NPC), cross-validated using multiple localization microscopy techniques, as well as two-color 3D reconstructions of the NPC, and reconstructions of DNA-origami tetrahedrons.